1) Cat. No.: TRC-A603260; 2) Chemical Name: N-(5-Amino-1-carboxypentyl)iminodiacetic Acid Tri-t-butyl Ester; 3) CAS No.: 205379-08-4; 4) Mol. Formula: C22H42N2O6; 5) Mol. Weight: 430.58; 6) Packing Size: 25mg / 250mg; 7) Appearence: Yellow Oil; 8) Application Notes: A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography.  This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag.  The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.

Also soluble in DMSO:DMF 1:1  and DMSO:CH2Cl2 1:2; 9) Synonym: N2,N2-Bis[2-(1,1-dimethylethoxy)-2-oxoethyl]-L-lysine 1,1-Dimethylethyl Ester; 10) Solubility: Chloroform, Methanol; 11) Storage condition: -20°C Freezer; 12) References: Hochuli, E., et al.: J. of Chromatography, 411, 177 (1987)